Molecular biology testing encompasses a diverse array of techniques meticulously employed to detect and analyze genetic information within biological samples.
These techniques harness a wide range of tools and methodologies, including gel electrophoresis, DNA amplification, and DNA sequencing.
The genetic information gleaned from this rigorous process finds applications in a multitude of fields, including disease diagnosis, identity determination and scientific research.
Our Molecular Biology Testing Services
Plant Identification
Molecular plant identification involves DNA extraction, amplification with PCR, sequencing, and comparison with databases to determine plant identity based on DNA sequences.
Animal Identification
Molecular animal identification involves extracting DNA from the sample of the animal to be identified, followed by amplification using PCR to multiply the specific target DNA sequences.
The DNA sequences are then analyzed through sequencing and compared with reference databases containing DNA sequences from various previously identified animal species.
Fungal Identification
Molecular fungal identification involves extracting DNA from the fungal sample, which is then amplified using PCR to multiply specific DNA sequences.
The resulting DNA sequences are analyzed using sequencing techniques, allowing determination of the nucleotide sequence within the DNA fragments.
The DNA sequence data are then compared with reference databases to accurately identify the type or species of fungus.
Bacterial Identification
Molecular bacterial identification involves DNA extraction, amplification with PCR, sequencing, and comparison with databases to determine bacterial species based on DNA sequences.
Gene Isolation
Gene isolation from various samples involves the extraction and separation of specific genes from the cells or tissues of an organism.
The process begins with DNA or RNA extraction from the sample. Afterward, the desired gene can be identified and isolated using techniques such as PCR or cloning with DNA vectors.
Toxin, Poison Detection, etc.
Molecular toxin detection is a technique that uses specific interactions between biological molecules and toxins to identify and measure toxins using methods such as PCR, qPCR, and ELISA, which allow amplification and detection of toxins through genetic material or proteins. Molecular biosensors, microarrays, and mass spectrometry are also used for sensitive and specific detection.
Gene Expression Analysis
Gene expression analysis is a technique to measure how active specific genes are in a cell or tissue by examining the amount of RNA produced from those genes.
This process reveals how genes are activated or deactivated under different conditions, such as in health versus disease or in response to drugs.
Commonly used methods include qPCR, which quantitatively measures RNA levels; microarrays, which can analyze the expression of thousands of genes simultaneously; and RNA-seq (RNA sequencing), which provides a comprehensive overview of the expression of all genes in a sample.
Protein Analysis (SDS-Page)
SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is a technique that separates proteins based on their size or molecular weight.
Proteins in a sample are treated with SDS to give them a negative charge and cause denaturation into linear chains.
When an electric current is applied to the polyacrylamide gel, the negatively charged proteins move towards the positive electrode. Smaller proteins move faster and farther through the gel, allowing separation by size.
After electrophoresis, the gel is stained to visualize the protein bands, which are analyzed to determine their relative sizes. SDS-PAGE is widely used for protein identification, purification, and study in research and industrial applications.
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